Bruker JPK NanoWizard® 4 XP Atomic Force Microscope (AFM)

The NanoWizard® 4 XP is an advanced Atomic Force Microscope (AFM) system that enables the combination of AFM-imaging/scanning experiments with optical microscopy. Thanks to its configuration, it supports the acquisition of several datasets in both biological and non-biological specimens. This includes the measurement of surface topography, elasticity, adhesion and deformation in air- or liquid-setup. The system is particularly suitable for the study of the topology and stiffness (Young’s Modulus) of living cells, but also morphology and biomechanical properties of tissues, biomaterials, isolated organelles or single molecules.

Key technical features:

-    JPK NanoWizard® 4 XP AFM scanner head: XYZ Tip-scanning technology, 100 × 100 × 15 µm3 scan range with 1.5 μm extra z range with high-speed option
-    infra-red (850 nm) deflection detection light source with low coherence
-    Imaging modes: PeakForce Tapping®, Contact mode with lateral force microscopy (LFM), Tapping

Mode™ with PhaseImaging™ the JPK QI™ Quantitative Imaging advanced mode:

-    Force measurements: force-distance spectroscopy, Advanced Force Mapping
-    JPK Manual Precision Stage with 20 × 20 mm2 travel range
-    JPK Vortis™2 SPM Controller
-    JPK SPMControl Software V7 including JPK DirectOverlay™ 2 software module, JPK QI™- Advanced 2 software module
-    JPK DP Data Processing (DP) Software

The AFM scanner head is combined with an inverted optical Olympus IX73 microscope that implements the imaging setup with:

-    Objective LCACHN 20X PH/0,4 WD 3.2mm;
-    Objective LCACHN 40X PH/0,55 WD 2.2mm;
-    long distance condenser (WD 73mm, NA 0.3, Brightfield, Ph1, Ph2);
-    1/1.2" IDS 3060 USB3.0 Color Camera

High resolution experiments can be achieved thanks to the combination with:

-    Accurion i4 Benchtop Active Vibration Isolation System
-    JPK acoustic enclosure and anti-vibration table.

Application examples:

Iriarte-Mesa C., Jobst M. Bergen J., Kiss E., Ryoo R., Kim J.-C., Crudo F., Marko D., Kleitz F., Del Favero G., Morphology-Dependent Interaction of Silica Nanoparticles with Intestinal Cells: Connecting Shape to Barrier Function, Nano Letters, July 11, 2023, DOI: 10.1021/acs.nanolett.3c00835

Gruber L., Jobst M., Kiss E., Karasová M., Englinger B., Berger W., Del Favero G., Intracellular remodeling associated with endoplasmic reticulum stress modifies biomechanical compliance of bladder cells, Cell Communication and Signaling, Oct. 30, 2023, DOI: 10.1186/s12964-023-01295-x